Laboratory of Biochemistry
Modification to tailor protein functionality
At NIZO, we have the tools and expertise to tailor the functionality of your proteins. We use enzymatic, physical and chemical modifications to achieve your goals.
To read more: http://www.nizo.com/explore/product-groups/protein-peptide-functionality/protein-modification
Contact expert Hans Kosters for more information: http://www.nizo.com/explore/people/91/koster/
Protein functionality in products
At NIZO, we apply state-of-the-art methodologies to evaluate hydrodynamic and surface functionalities.
The results can be used to improve sensory, visual, textural, rheological and surfactant properties.
To read more: http://www.nizo.com/explore/product-groups/protein-peptide-functionality/functionality-in-products
Contact expert Saeed Rahimi Yazdi for more information: http://www.nizo.com/explore/people/156/rahimi-yazdi/
Mission: GBB aims to excel in interdisciplinary fundamental biomolecular research on prokaryotic and eukaryotic microorganisms, which comprise the richest biological reservoir of metabolic diversity on the planet. GBB advances on new biotechnological applications through molecular- and synthetic biology approaches, offers a stimulating environment for research and training, and maintains state-of-the-art infrastructure.
Profile: With strong roots in chemistry, biophysics and biology, GBB has become a well-recognized research institute in biomolecular science with a focus on molecular microbiology and structure-function studies of biomolecules. In recent years, GBB has seen a remarkable growth in microbiological sciences, stimulated by strong teams incorporating modern approaches in genomics, single cell- and single molecule analyses, high-resolution microscopy, and advanced cell engineering with synthetic biology approaches incorporated. In view of the emerging societal and industrial need to develop sustainable bio-based processes, and the undisputed and important role that microbial processes play in this field, GBB strives to further concentrate its research profile in two focal areas: Molecular Mechanisms of Bioprocesses and Physiology and Systems Biology.
GBB has the critical mass of theoretical and experimental research directions, unique facilities, and an internationally recognized staff to embark on innovation pipelines as part of the Protein Competence Centre.
In vitro Enzymatic Hydrolysis
Metagenomics combined with dedicated engineering (directed evolution, mutagenesis, synthetic biology) of (novel) industrially relevant enzymes with emphasis on sustainable applications in carbohydrate bioprocessing, fine chemical production, and societal relevant conversion processes for production of (functionalized) added value proteins. Proteolysis based conversions of proteinaceous feedstocks exploiting the (engineered) Bacillus proteases.
Various contributors to larger PPS programmes focused on challenges in the biobased economy (CCC, BE-Basic, TA-COAST, EU projects, TKI BBE, TKI Agro/Food)
Synthetic Biology and Advanced (metabolic) Engineering of Industrial Microorganisms
Genetic, molecular, structural and biophysical analysis of membranes and membrane proteins (e.g. peptide and solute transporters) in industrial microorganisms such as those that are relevant for industrial fermentation, food conservation and safety and for flavor development. Systems biology and engineering of (industrial) microorganisms (yeast, bacteria) using synthetic biology approaches are fully encountered.
Protein Structure and Functionality
Modelling and simulation of dynamics of proteins and larger protein complexes. Expert and internationally renowned for development and use of molecular dynamic simulation tools (e.g. GROMOS, GROMACS, MARTINI)
High-resolution structure determination of proteins and protein complexes, which are relevant for applications in biotechnology (biocatalysts), food (e.g. carbohydrate modifying enzymes) and health (host invasion vectors). Expert in protein X-ray crystallization of individual proteins and proteins with trapped ligands/substrates and access to high-end X-ray beamlines at synchrotron facilities in Europe.
Structure determination of complex biomolecular assemblies by using HR cryo TEM for single particle analysis and electron tomography (both combined with extensive data processing) with the aim to get insight into structure and function membrane proteins and larger biomacromolecules. Partner in and user of NECEN facilities.
Smart Fermentation of Proteinaceous Feedstocks
Systems biology and cell wall/membrane engineering of industrial microorganisms in conjunction to optimized protein secretion and global regulatory pathways for smart fermentation of bulk proteinaceous feedstocks to convert raw protein feedstocks (via growth) towards production of dedicated functionalized and/or added value proteins/peptides and building blocks. BBE projects funded by e.g. STW, Be-Basic, Kluyver Centre, and EU-ITN are ongoing.